Designing libraries of chimeric proteins using SCHEMA recombination and RASPP.
نویسندگان
چکیده
SCHEMA is a method for designing libraries of novel proteins by recombination of homologous sequences. The goal is to maximize the number of folded proteins while simultaneously generating significant sequence diversity. Here, we use the RASPP algorithm to identify optimal SCHEMA designs for shuffling contiguous elements of sequence. To exemplify the method, SCHEMA is used to recombine five fungal cellobiohydrolases (CBH1s) to produce a library of more than 390,000 novel CBH1 sequences.
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عنوان ژورنال:
- Methods in molecular biology
دوره 1179 شماره
صفحات -
تاریخ انتشار 2014